Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar.
Identifieur interne : 002B09 ( Main/Exploration ); précédent : 002B08; suivant : 002B10Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar.
Auteurs : John Ralph [États-Unis] ; Takuya Akiyama [Japon] ; Heather D. Coleman [États-Unis] ; Shawn D. Mansfield [Canada]Source :
- Bioenergy research [ 1939-1234 ]
Abstract
The lignin structural ramifications of coumarate 3-hydroxylase (C3H) downregulation have not been addressed in hardwoods. Such information is required to accompany an assessment of the digestibility and bioenergy performance characteristics of poplar, in particular. Structurally rich 2D NMR methods were applied to the entire lignin fraction to delineate lignin p-hydroxyphenyl:guaiacyl:syringyl (H:G:S) levels and linkage distribution changes (and to compare with traditional degradative analyses). C3H downregulation reduced lignin levels by half and markedly increased the proportion of H units relative to the normally dominant G and S units. Relative stem H unit levels were up by ∼ 100-fold to ∼ 31 %, almost totally at the expense of G units; differences in the lignin interunit linkage distributions were more subtle. The H level in the most drastically C3H-downregulated transgenic poplar falls well beyond the H:G:S compositional bounds of normal angiosperms. The response observed here, in poplar, differs markedly from that reported for alfalfa where the S:G ratio remained almost constant even at substantial H levels, highlighting the often differing responses among plant species.
DOI: 10.1007/s12155-012-9218-y
PubMed: 26366246
PubMed Central: PMC4560085
Affiliations:
- Canada, Japon, États-Unis
- Colombie-Britannique , Région de Kantō, Wisconsin, État de New York
- Tokyo, Vancouver
- Université de Tokyo, Université de la Colombie-Britannique
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Coleman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Syracuse University, 460 Life Sciences Complex, 107 College Place, Syracuse, NY 13244 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Biology, Syracuse University, 460 Life Sciences Complex, 107 College Place, Syracuse</wicri:cityArea></affiliation></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC V6T 1Z4 Canada.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></analytic><series><title level="j">Bioenergy research</title><idno type="ISSN">1939-1234</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The lignin structural ramifications of coumarate 3-hydroxylase (C3H) downregulation have not been addressed in hardwoods. Such information is required to accompany an assessment of the digestibility and bioenergy performance characteristics of poplar, in particular. Structurally rich 2D NMR methods were applied to the entire lignin fraction to delineate lignin <i>p</i>-hydroxyphenyl:guaiacyl:syringyl (H:G:S) levels and linkage distribution changes (and to compare with traditional degradative analyses). C3H downregulation reduced lignin levels by half and markedly increased the proportion of H units relative to the normally dominant G and S units. Relative stem H unit levels were up by ∼ 100-fold to ∼ 31 %, almost totally at the expense of G units; differences in the lignin interunit linkage distributions were more subtle. The H level in the most drastically C3H-downregulated transgenic poplar falls well beyond the H:G:S compositional bounds of normal angiosperms. The response observed here, in poplar, differs markedly from that reported for alfalfa where the S:G ratio remained almost constant even at substantial H levels, highlighting the often differing responses among plant species.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">26366246</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1939-1234</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><Issue>4</Issue><PubDate><MedlineDate>2012</MedlineDate></PubDate></JournalIssue><Title>Bioenergy research</Title><ISOAbbreviation>Bioenergy Res</ISOAbbreviation></Journal><ArticleTitle>Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar.</ArticleTitle><Pagination><MedlinePgn>1009-1019</MedlinePgn></Pagination><Abstract><AbstractText>The lignin structural ramifications of coumarate 3-hydroxylase (C3H) downregulation have not been addressed in hardwoods. Such information is required to accompany an assessment of the digestibility and bioenergy performance characteristics of poplar, in particular. Structurally rich 2D NMR methods were applied to the entire lignin fraction to delineate lignin <i>p</i>-hydroxyphenyl:guaiacyl:syringyl (H:G:S) levels and linkage distribution changes (and to compare with traditional degradative analyses). C3H downregulation reduced lignin levels by half and markedly increased the proportion of H units relative to the normally dominant G and S units. Relative stem H unit levels were up by ∼ 100-fold to ∼ 31 %, almost totally at the expense of G units; differences in the lignin interunit linkage distributions were more subtle. The H level in the most drastically C3H-downregulated transgenic poplar falls well beyond the H:G:S compositional bounds of normal angiosperms. The response observed here, in poplar, differs markedly from that reported for alfalfa where the S:G ratio remained almost constant even at substantial H levels, highlighting the often differing responses among plant species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Enzyme Institute, University of Wisconsin-Madison, 1710 University Avenue, Madison, WI 53726 USA ; Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706 USA ; DOE Great Lakes Bioenergy Research Center, and Wisconsin Bioenergy Initiative, University of Wisconsin-Madison, Madison, WI 53706 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Akiyama</LastName><ForeName>Takuya</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Wood Chemistry Laboratory, Department of Biomaterial Sciences, the University of Tokyo, Bunkyo-ku Tokyo, 113-8657 Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Coleman</LastName><ForeName>Heather D</ForeName><Initials>HD</Initials><AffiliationInfo><Affiliation>Department of Biology, Syracuse University, 460 Life Sciences Complex, 107 College Place, Syracuse, NY 13244 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, Vancouver, BC V6T 1Z4 Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P41 RR002301</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>S10 RR002781</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>S10 RR008438</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Bioenergy Res</MedlineTA><NlmUniqueID>101491631</NlmUniqueID><ISSNLinking>1939-1234</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biomass conversion</Keyword><Keyword MajorTopicYN="N">Digestibility</Keyword><Keyword MajorTopicYN="N">Gene downregulation</Keyword><Keyword MajorTopicYN="N">Lignin composition</Keyword><Keyword MajorTopicYN="N">NMR</Keyword><Keyword MajorTopicYN="N">Thioacidolysis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>9</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26366246</ArticleId><ArticleId IdType="doi">10.1007/s12155-012-9218-y</ArticleId><ArticleId IdType="pii">9218</ArticleId><ArticleId IdType="pmc">PMC4560085</ArticleId></ArticleIdList><pmc-dir>pmcsd</pmc-dir>
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IdType="pubmed">18316744</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>Japon</li><li>États-Unis</li></country><region><li>Colombie-Britannique </li><li>Région de Kantō</li><li>Wisconsin</li><li>État de New York</li></region><settlement><li>Tokyo</li><li>Vancouver</li></settlement><orgName><li>Université de Tokyo</li><li>Université de la Colombie-Britannique</li></orgName></list><tree><country name="États-Unis"><region name="Wisconsin"><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name></region><name sortKey="Coleman, Heather D" sort="Coleman, Heather D" uniqKey="Coleman H" first="Heather D" last="Coleman">Heather D. Coleman</name></country><country name="Japon"><region name="Région de Kantō"><name sortKey="Akiyama, Takuya" sort="Akiyama, Takuya" uniqKey="Akiyama T" first="Takuya" last="Akiyama">Takuya Akiyama</name></region></country><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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